Genetic Data for Typha species in the Great Lakes Region (National Parks)

Metadata:
Identification_Information:
Citation:
Citation_Information:
Originator: U.S. Geological Survey National Wetlands Research Center
Publication_Date: unpublished material
Title:
Genetic Data for Typha species in the Great Lakes Region (National Parks)
Geospatial_Data_Presentation_Form: digital tabular data
Description:
Abstract:
The rapid and ongoing spread of cattails (Typha spp.) in the national parks of the Great Lakes region poses a serious threat to biodiversity and ecosystem function. Although nutrient loading and hydrology have been cited as possible causations in this invasion, an equally plausible explanation is an expansion of the ecological tolerances of this taxon through hybridization. Typha glauca is believed to represent a hybrid between the native North American cattail (Typha latifolia), and a naturalized species originating from Europe, Typha angustifolia. This conclusion is based primarily on morphological evidence, although cross-breeding experiments have confirmed the ability of T. latifolia and T. angustifolia to hybridize. This data set consists of applications of modern molecular techniques to determine the actual extent of hybridization across this region.
Purpose:
To verify through genetic analysis the presence (or absence) of hybrid cattails in three Great Lakes National parks units (Indiana Dunes National Lakeshore, St. Croix National Scenic Riverway, and Voyageurs National Park) and to determine the relative proportions of T. latifolia, T. angustifolia, and T. glauca in each unit.
Time_Period_of_Content:
Time_Period_Information:
Range_of_Dates/Times:
Beginning_Date: 2004
Ending_Date: 2006
Currentness_Reference: ground condition
Status:
Progress: In work
Maintenance_and_Update_Frequency: none planned
Spatial_Domain:
Bounding_Coordinates:
West_Bounding_Coordinate: -93.180
East_Bounding_Coordinate: -86.900
North_Bounding_Coordinate: 48.625
South_Bounding_Coordinate: 41.625
Keywords:
Theme:
Theme_Keyword_Thesaurus: None
Theme_Keyword: cattail
Theme_Keyword: hybridization
Theme_Keyword: invasive
Theme_Keyword: wetlands
Theme_Keyword: Great Lakes
Theme_Keyword: genetics
Theme_Keyword: genetic analysis
Theme_Keyword: relation
Theme_Keyword: AFLP
Theme_Keyword: amplified fragment length polymorphism
Theme_Keyword: DNA
Theme_Keyword: hybrid
Place:
Place_Keyword_Thesaurus: None
Place_Keyword: Great Lakes
Place_Keyword: Indiana
Place_Keyword: Indiana Dunes National Lakeshore
Place_Keyword: St. Croix National Scenic Riverway
Place_Keyword: Voyageurs National Park
Place_Keyword: Minnesota
Place_Keyword: Wisconsin
Place_Keyword: MN
Place_Keyword: IN
Place_Keyword: WI
Place_Keyword: Apostle Islands National Park
Place_Keyword: Isle Royale National Park
Place_Keyword: Michigan
Place_Keyword: MI
Taxonomy:
Keywords/Taxon:
Taxonomic_Keyword_Thesaurus: None
Taxonomic_Keywords: Typha latifolia
Taxonomic_Keywords: Typha angustifolia
Taxonomic_Keywords: Typha glauca
Taxonomic_Keywords: species
Taxonomic_Classification:
Taxon_Rank_Name: Kingdom
Taxon_Rank_Value: Plantae
Taxonomic_Classification:
Taxon_Rank_Name: Division
Taxon_Rank_Value: Magnoliophyta
Taxonomic_Classification:
Taxon_Rank_Name: Class
Taxon_Rank_Value: Liliopsida
Taxonomic_Classification:
Taxon_Rank_Name: Subclass
Taxon_Rank_Value: Commelinidae
Taxonomic_Classification:
Taxon_Rank_Name: Order
Taxon_Rank_Value: Typhales
Taxonomic_Classification:
Taxon_Rank_Name: Family
Taxon_Rank_Value: Typhaceae
Taxonomic_Classification:
Taxon_Rank_Name: Genus
Taxon_Rank_Value: Typha
Taxonomic_Classification:
Taxon_Rank_Name: Species
Taxon_Rank_Value: latifolia
Applicable_Common_Name: common cattail
Taxonomic_Classification:
Taxon_Rank_Name: Species
Taxon_Rank_Value: angustifolia
Applicable_Common_Name: narrowleaf cattail
Taxonomic_Classification:
Taxon_Rank_Name: Species
Taxon_Rank_Value: glauca
Applicable_Common_Name: white cattail
Access_Constraints:
Any downloading and use of these data signifies a user's agreement to comprehension and compliance of the NWRC Standard Disclaimer. Insure all portions of metadata are read and clearly understood before using these data in order to protect both user and NWRC interests. See section 6.3 Distribution Liability.
Use_Constraints:
Acknowledgement of the National Wetlands Research Center as a data source would be appreciated in products developed from these data, and such acknowledgement as is standard for citation and legal practices for data source is expected by users of this data. Sharing new data layers developed directly from these data would also be appreciated by NWRC staff. Users should be aware that comparisons with other data sets for the same area from other time periods may be inaccurate due to inconsistencies resulting from changes in analyses and digital processes over time. These data are not legal documents and are not to be used as such.
Point_of_Contact:
Contact_Information:
Contact_Organization_Primary:
Contact_Organization: U.S. Geological Survey, National Wetlands Research Center
Contact_Person: Steven Travis
Contact_Position: Ecologist
Contact_Address:
Address_Type: Mailing and Physical
Address: 700 Cajundome Blvd
City: Lafayette
State_or_Province: LA
Postal_Code: 70506
Country: USA
Contact_Voice_Telephone: 337 266-8583
Contact_Facsimile_Telephone: 337 266-8592
Contact_Electronic_Mail_Address: steven_travis@usgs.gov
Analytical_Tool:
Analytical_Tool_Description:
ABI Prism 310 Genetic Analyzer: performs a variety of applications such as comparative sequencing, linkage analysis, STR analysis, SNP detection, discovery and validation, and mutation detection.
Tool_Access_Information:
Online_Linkage: <http://www.appliedbiosystems.com>
Tool_Access_Instructions: See website for information
Tool_Contact:
Contact_Information:
Contact_Organization_Primary:
Contact_Organization: Applied Biosystems, Inc
Contact_Address:
Address_Type: Physical
Address: 850 Lincoln Centre Drive
City: Foster City
State_or_Province: CA
Postal_Code: 94404
Country: USA
Contact_Voice_Telephone: 800 327-3302
Contact_Facsimile_Telephone: 650 638-5800
Data_Set_Credit:
The National Wetlands Research Center would like to acknowledge the National Park Service for their participation in this study.
Native_Data_Set_Environment: unknown
Cross_Reference:
Citation_Information:
Originator: Kuehn, M.M., J.E. Minor, and B.N. White
Publication_Date: 1999
Title:
An examination of hybridization between the cattail species Typha latifolia and Typha angustifolia using random amplified polymorphic DNA and chloroplast DNA markers
Geospatial_Data_Presentation_Form: journal article
Publication_Information:
Publisher: Blackwell Publishing
Publication_Place: Malden, MA
Series_Information:
Series_Name: Molecular Ecology
Issue_Identification: 8(12):1981-1990
Cross_Reference:
Citation_Information:
Originator:
Tsyusko-Omeltchenko, O.V., N.A. Schable, M.H. Smith, and T.C. Glenn
Publication_Date: 2003
Title:
Microsatellite loci isolated from narrow-leaved cattail Typha angustifolia
Geospatial_Data_Presentation_Form: journal article
Publication_Information:
Publisher: Blackwell Publishing
Publication_Place: Malden, MA
Series_Information:
Series_Name: Molecular Ecology
Issue_Identification: 12(3):535-538
Data_Quality_Information:
Attribute_Accuracy:
Attribute_Accuracy_Report:
The accuracy of the attributes is dependent on the instrumentation and statistical software used for analyses. An exact accuracy value can not be determined due to the procedures and software used.
Logical_Consistency_Report:
Will be reported once study is complete. Processes and methodology may change during the course of research.
Completeness_Report:
Initially the study sampled from three national parks (Indiana Dunes National Seashore, St. Croix National Scenic Riverway, and Voyageurs National Park). The study expanded to four more national parks (Apostle Islands National Lakeshore, Fire Island National Seashore, Assateague Island National Seashore, and Isle Royale National Park) to develop species diagnostic markers; two parks were identified as harboring isolated populations. The completeness of these data is currently unknown since the project is still ongoing.
Positional_Accuracy:
Horizontal_Positional_Accuracy:
Horizontal_Positional_Accuracy_Report:
The accuracy of the horizontal postitions is dependent on the GPS unit used to gather coordinates and any post-processing activity conducted on them.
Lineage:
Methodology:
Methodology_Type: Field
Methodology_Identifier:
Methodology_Keyword_Thesaurus: None
Methodology_Keyword: sampling
Methodology_Keyword: leaf tissue
Methodology_Keyword: morphology
Methodology_Keyword: transect
Methodology_Keyword: leaf width
Methodology_Keyword: plant height
Methodology_Keyword: spike morphology
Methodology_Keyword: flowering stage
Methodology_Keyword: GPS
Methodology_Keyword: coordinates
Methodology_Description:
Sample collection and preparation - From sites chosen on morphological distinction among the region's national parks, samples were collected. Leaf tissue was removed from each plant and maintained on ice prior to freezing at -20 degrees Celsius for subsequent genetic analysis. For each Typha clone sampled, leaf width, plant height, spike morphology, and flowering stage was recorded.
Methodology:
Methodology_Type: Lab
Methodology_Identifier:
Methodology_Keyword_Thesaurus: None
Methodology_Keyword: AFLP
Methodology_Keyword: amplified fragment length polymorphism
Methodology_Keyword: markers
Methodology_Keyword: primer
Methodology_Keyword: multi-locus
Methodology_Keyword: DNA fingerprinting
Methodology_Description:
Genetic analysis - To genetically characterize hybrids, the species-diagnostic RAPD (randomly amplified polymorphic DNA) markers of Kuehn et al. (1999) were used. A more detailed assessment of levels of admixture within individuals identified as hybrids will rely on the microsatellite loci described in Tsyusko-Omeltchenko et al. (2003).
Process_Step:
Process_Description: See Methodology
Process_Date: 2004-2006
Entity_and_Attribute_Information:
Overview_Description:
Entity_and_Attribute_Overview:
The attributes in this study were measured and calculated for the purpose of verifying through genetic analysis the presence (or absence) of hybrid cattails in three Great Lakes National parks units (Indiana National Lakeshore, St. Croix National Scenic Riverway, and Voyageurs National Park) and determining the relative proportions of T. latifolia, T. angustifolia, and T. glauca in each unit
Entity_and_Attribute_Detail_Citation: USGS Project number 3210BG4 Task 16 report
Metadata_Reference_Information:
Metadata_Date: 20060213
Metadata_Contact:
Contact_Information:
Contact_Organization_Primary:
Contact_Organization: U.S. Geological Survey, National Wetlands Research Center
Contact_Position: Metadata Specialist
Contact_Address:
Address_Type: Mailing and Physical
Address: 700 Cajundome Blvd
City: Lafayette
State_or_Province: LA
Postal_Code: 70506
Country: USA
Contact_Voice_Telephone: 337 266-8500
Contact_Facsimile_Telephone: 337 266-8513
Metadata_Standard_Name: Content Standard for Digital Geospatial Metadata
Metadata_Standard_Version: FGDC-STD-001-1998
Metadata_Access_Constraints: None
Metadata_Use_Constraints:
Acknowledgement of the National Wetlands Research Center as the metadata source would be appreciated. Please cite the original metadata when using portions to create another record for slightly altered data, such as reprojection.
Generated by mp version 2.7.33 on Mon Feb 13 13:42:49 2006